Method for Determining Bromate Content in Flour, Bread, and Drinking Water Using Anyeep TQ9100
This study utilized the Anyeep TQ9100 triple quadrupole mass spectrometer from Anyeep Company to develop a method for detecting bromate in flour, bread, and drinking water. The method demonstrates excellent linearity, with detection limits and quantification limits meeting relevant standard requirements. Using this method and equipment enables the detection and quantification of bromate in food and drinking water.
Bromate is widely used as a baking additive in bread production, where it acts as a slow oxidizing agent. It also forms as a byproduct when natural water sources such as mineral water and spring water are disinfected with ozone.
However, bromate is classified as a Group 2B probable human carcinogen internationally. In Announcement No. 9 issued in 2005, the Ministry of Health mandated the discontinuation of potassium bromate as a flour treatment agent in wheat flour. The "GB 5749-2022 Standard for Hygiene of Drinking Water" requires that bromate levels in drinking water remain below 0.01mg/L.
This method employs ion chromatography-tandem mass spectrometry (IC-MS/MS). Sample pretreatment for wheat flour, bread, and drinking water follows GB/T 20188-2006, SN/T 3138-2012, and GB/T 5750.10-2023. Bromate content is quantified using the external standard method in accordance with GB/T 5750.10-2023.
Instruments and Reagents
Anyeep TQ120 Ultra-High Performance Liquid Chromatography-Tandem Mass SpectrometrySystem
OIC-1100 Ion Chromatograph
OEDG-100 (KOH Type)
Dionex ADRS 600 2 mm Anion Suppressor (Hebei Ourun Scientific Instrument Co., Ltd.)
RP Pre-treatment Column and Ba/Ag/H Pre-treatment Column (Hebei Ouren Scientific Instrument Co., Ltd.)
Liquid Chromatography Conditions
Mobile Phase: KOH eluent
Chromatography column: Dionex IonPac™ AS11-HC 2×250 mm
Column Temperature：40 ℃
Flow rate: 0.3 mL/min
Injection volume：100 μL
Mass Spectrometry Conditions
Ionization Source： Electrospray Ionization Source (ESI)-）
Source Temperature： 550 ℃
Spray Voltage： 4500 V
air curtain： 20 psi
Mist gas： 50 psi
Auxiliary Gas： 50 psi
Crash： 6
Scan Mode: SIM/MRM
Sample Processing
Samples are processed according to GB/T 5750.10-2023.
Summary
This document establishesIon Chromatography -Detection method for bromate content in bread, flour, and drinking water using the tandem mass spectrometry system (Anyeep TQ9100). Based on the resultszhAs shown,ThisMethodBoth SIM and MRM modes can be used for analysis. Each mode meets the relevant standard requirements, with MRM offering superior sensitivity compared to SIM. This methodOffers excellent sensitivity, stability, and linear range; suitable forBromate levels in different samplesRoutine analysis and testing.